Abstract
The detection of environmental pollutants has challenged conventional chemical analysis for the rapid estimation of the
bioavailability and toxicity of the contamination impact on human health and ecosystems. Magnetic nanoparticles (MNPs)
functionalization as bioreporters can enhance the ability for biosensing in complex contaminated samples such as soils, and the
immobilization of bioreporters will promote the detection of gas and online monitoring. In this study, Acinetobacter baylyi ADP1
bioreporters ADPWH_lux and ADPWH_recA were used to evaluate the optimization of MNPs bioreporter functionalization and
filter immobilization. It demonstrated that the MNPs functionalized and filter-immobilized bioreporters retained a similar
sensitivity to the original non-treated bioreporters. These approaches will provide new ways for the detection of contaminants in
soil and air samples, with prospects for in situ and online measurement. This study also suggested that portable application and
miniaturization is possible when the functionalized bioreporter is immobilized on a filter or magnetic probe.