Abstract
Hydrolysates of Arundo donax, a crop offering high productivity in contaminated or salinized soils with no inputs of irrigation
and agrochemicals, were used in a discontinuous fermenter to grow the oleaginous yeast Lipomyces starkey, to obtain microbial
oils potentially useful for the production of 2nd-generation biodiesel.
A mixture of fermentable sugars was obtained by steam-explosion and subsequent enzymatic hydrolysis of the lignocellulosic
materials. The concentration of Ni2+ ions and of inhibitors of the microbial growth significantly affected both the biomass and the
triglyceride yields. The microbial lipids produced were compatible with the synthesis of an automotive-grade biodiesel.
A physico-mathematical model, developed to describe the biomass growth, demonstrated that the concentration of heavy metals
affected the maximum biomass concentration, though its influence on the specific growth rate of the yeasts was not significant.