An aliphatic-amine-bearing chromatographic support, based on commercial silica, was prepared and tested as potential matrix for affinity immobilization of a copper/topaquinone-containing amine oxidase from lentil seedlings. The support proved to be noticeably effective in immobilizing the enzyme with concomitant purification and activity retention. The immobilization yields and some properties of this preparation were also determined and compared with those of the soluble enzyme. High activity r...
Autori: Barbara Noli, Enrico Sanjust, Francesca Sollai, Giovanni Floris
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=25108

AbstractDark fermentation hydrogen production from a mixture of food components using two different methods of methanogenic inhibition (autoclaving and BES) and three different temperatures (37, 60, and 70 Â?C) was examined in batch assays for two different mixed anaerobic cultures - one suspended sludge (S) obtained from an anaerobic digester and one granular sludge (G) obtained from a brewery wastewater treatment plant. In general, BES-inhibition of sludge was more robust when compared against...
Autori: Angela A. Abreu, Anthony S. Danko, Francisco Pinheiro, Maria Madalena Alves
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=25308

AbstractChemical sensors utilizing immobilized enzymes and proteins are important for monitoring chemical processes and biological systems. Glucose oxidase (GOx) from Aspergillum Niger is a flavoenzyme that has been used to determine glucose. Among the conventional methods of enzyme deposition the main strategy consists in its entrapment in an inert matrix adsorbed on the electrode surface. We report here the synthesis and physical – chemical characterization of a new bionanocomposites obtaine...
Autori: , Gabriela Ciobanu, Ion Balasanian, Simona Ratoi
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=25359

AbstractPulsed high voltage discharge technology is a newly developed method for knock out viruses in blood with various electrohydraulic discharge reactors. The aim of this study is to investigate the effect of an electrohydraulic discharge treatment system on Hepatitis C virus (HCV) behaviour. The electrohydraulic discharge (EHD) reactor consist of high voltage point discharge electrode above blood surface and cylindrical ground copper electrode containing the blood (in the same time act as th...
Autori: Gamal M. El-Aragi
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=25362

Abstract Molecular imprinting is a versatile and ecological method for obtaining polymeric materials with a lot of applications in the field of pharmacy, medicine and biosensor assays. In this work, a new method of alternative molecular imprinting to design imprinted poly (vinyl alcohol) films has been proposed. Glycyrrhizinic acid has been used as template molecule. To demonstrate the obtaining of the molecular imprinted poly (vinyl alcohol) and its selectivity, studies of glycyrrhizinic and...
Autori: , Catalin Croitoru, Georgeta Moise
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=25495

Abstract It is known that carbonhydrates are precursors of all bioactive substances we tried in thes paper work to do some investigation on these substances in the environmental area of Iasi. To observe the influence of some chemical mutagens substances on carbohydrates biosynthesis we conducted investigations on plant material (leaves) taken from the Calendula officinalis L species. We used this assay to examine the effects of the herbicide 2,4- dichlorophenoxyacetic acid (2,4-D), etidium br...
Autori: Constantin Leonte, Roxana Vatavu, Teodor Robu, Irina Volf
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=25502

Abstract The use of biomaterials as biosorbents for treatment of wastewaters will provide as a potential alternate to the conventional treatment methods. The main advantages of biosorption are high selectivity , cost effectiveness and good removal performance. World wide numerous low cost bio materials have tried for biosorption of dyes. In this present investigation, the dead biomass of Penicillium sp. fungus and wood sawdust was used as biosorbents and their capacity to removed malachite gr...
Autori: Cornel Pohontu, Florin Luca, Igor Creţescu, Manea Liliana, Mariana Diaconu
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=25593

Abstract Two different inocula - acclimated and non-acclimated to fat- were used to evaluate the methane production of cow manure, food waste and oily waste in batch assays. The inoculum adapted to fat had a better performance in the methanisation of substrates with significant lipids content. Furthermore, it is demonstrated that an increase in the ratio inoculum/substrate can enhance the initial methane production rate of oily waste when using a non-adapted inoculum, improving also the ultim...
Autori: Lucia Neves, Maria Madalena Alves, Raquel Ferreira, Rosario Oliveira
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=25634

Abstract The aim of this work was the evaluation of the denitrification potential of a consortium of activated sludge adapted to anoxic conditions. As the carbon source is the main parameter influencing the denitrification process, in the first stage three carbon sources (acetate, citrate and glucose) were assayed in batch activity tests. The highest denitrification efficiency was ttained with acetate, being nitrate and nitrite completely reduced. The adapted sludge was then used as a potent...
Autori: Joana Azeredo, Pilar Teixeira, Rosario Oliveira, Zelia Fernandes
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=25638

? Environmental Engineering and Management Journal, Vol. 9 Nr. 4 ? THE PROTECTION OF EQUIPMENTS USING VOLATILE INHIBITORS ? CONTINUOUS SURFACE TEMPERATURE MONITORING TO ESTIMATE SENSIBLE HEAT LOSS BY BUILDING FINISHES ? RECYCLE OF POLYAMIDE DYEING WASTEWATER FOLLOWING DECOLORIZATION WITH POLYMERIC SORBENTS ? PERFORMANCE EVALUATION OF AMINO ACID SALT-BASED COMPLEX ABSORBENTS FOR CO2 CAPTURE ? ADSORPTION EQUILIBRIUM STUDIES ON COPPER (II) IONS REMOVAL BY NATURAL WASTE USIN...
Autori:
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=25666

Rhizobag incubation experiments were conducted to study microbial biomass carbon (MBC), microbial biomass nitrogen (MBN), soil respiration and enzyme activity (urease and phosphatase) in the rhizosphere soil of three different wetland plants and its influence on the removal of total organic carbon (TOC) and total nitrogen (TN). MBC, MBN, soil respiration and enzyme activity were higher in the rhizosphere than in the non-rhizosphere soil of the wetland plants. Microbial activity in the rhizospher...
Autori: Defu Xu, Hua Fang, Xianghua Xu, Xiaoli Zhao, Yingxue Lihttp://www.ecozone.ro/reviste.php?RID=25679

Abstract: The paper focuses on eutrophication due to nutrients load into a reservoir. A thermally stratified reservoir water body is divided in vertical layers and horizontal zones according to water temperature and depth. The layers show different compositions of limiting nutrient because of the density of flow and due to inhabitants on the bottom of the reservoir exposed to sunlight. The bioassay for algal growth potential test (AGPT) is the most direct and effective way to determine a limitin...
Autori: Dimitri Solomatine, Ioana Popescu, Jae-Ki Shin, Nahm-Chung Jung, Peter Kelderman, Roland K. Price
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=25798

Abstract:Alkaline protease from Conidiobolus coronatus which is optimally active at pH 10 and 40°C finds application in leather and detergent industries as well as for recovery of silver from waste photographic films. The protease was immobilized in Ca-alginate, polyacrylamide gel and alkali treated waste fungal biomass (ATWFB). ATWFB was found to be most suitable among the matrices tested. Glutaraldehyde marginally increased the binding to ATWFB. Binding of protease to ATWFB seems to be through...
Autori: Ryali Seeta Laxman, Shiv Shankar
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=25807

The aim of this work is the study of activated sludge floc samples stained with different dyes and imaged by Confocal Laser Scanning Microscopy (CLSM). Activated sludge flocs were stained for lipids, sugars and esterase enzyme producing bacteria. Images were acquired for different samples to analyze the structural characteristics of the bioaggregates. The analysis of the CLSM images allowed for the localization and estimation of the quantity of different build-up materials. The 3-D reconstructio...
Autori: Bela Abraham, Botond Raduly, Dan Niculae Robescu, Szabolcs Lanyi, Szabolcs Szilveszter, Szilard Bucs
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=25937

The first part of this work presents the development and implementation in the Benchmark Simulation Model No 1 (BSM1) of a modified Activated Sludge Model No 3 (ASM3). The enhanced ASM3 presented in this study has three modifications compared to the original ASM3. The first modification is the representation of the simultaneous heterotrophic biomass growth on the primary substrate and on the internal storage products. Second, nitrification is modeled as a two-step process with nitrite as an inte...
Autori: George Simion Ostace, Paul Şerban Agachi, Vasile Mircea Cristea
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=25865

AbstractThe huge emission of greenhouse gases from fossil-fuelled power plants is emphasizing the need for efficient Carbon Captureand Storage (CCS) technologies. The biomimetic CO2 absorption in aqueous solutions has been recently investigated as apromising innovative alternative for post-combustion CCS. The carbonic anhydrase (CA) - a broad group of ubiquitous enzymes– may catalyse the CO2 hydration reaction and then to promote CO2 absorption rate into aqueous solutions. Nevertheless theresear...
Autori: Antonio Marzocchella, Giuseppe Olivieri, Maria Elena Russo, Piero Salatino
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=25976

AbstractEast Calcutta Wetland ecosystem is a Ramsar Site in India covering almost 51 km2 and is one of the largest of its type. Thiswetland receives a massive load of almost untreated industrial and domestic effluents every day, throughout the year, that hasbeen effectively utilized in pisciculture for having a better yield (2.85 metric tons of fish per 0.01 km2 of water body per annum).The present investigation was carried out to study the toxicity of the untreated effluents discharged from tan...
Autori: Utpal Singha Roy
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=26094

The book edited by Haibo Xie and NicholasGathergood focuses on one of the most importantscientific issues of the 21 century which is thereplacing fossil fuel with sustainable alternatives suchas biomass. Fossil fuels are conventional rawmaterials for many industrial sectors includingchemical and energy production. Usage of fossil fuelis limited by their availability, price and carbonfootprint. Biomass is a renewable resource globallyavailable, cheaper and carbon sequestering. Step bystep shiftin...
Autori: Adrian Catalin Puitel
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=26101

AbstractThe efficiency of enzymatic hydrolysis of Penicillin G to 6-Aminopenicillanic acid under substrate inhibition has been analyzedfor a bioreactor with mobile bed of immobilized penicillin amidase. The results indicated that the optimum values of temperatureand pH remained the same as for homogeneous hydrolysis using free enzyme. The inhibitory effect induced at higher PenicillinG amount in liquid phase was attenuated by increasing the size of the biocatalyst particles. For this reason, at ...
Autori: Ramona Mihaela Matran
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=26224

AbstractWastewater sludges are organic and nutrient-rich materials that could be used to improve soil tilth. However, the land applicationof sludge needs to be properly managed to avoid the detrimental effects on micro and macroorganisms in the soil. Earthwormsare one of the most important soil macroorganisms. Addition of sludge to the soil environment may impact earthworm activity,and in turn, soil productivity and health. In this study, sludge from the canned food industry was added to soil mi...
Autori: Efsun Dindar
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=26238

AbstractSeed priming has been reported to be a simple technique for enhancing seedling establishment in environment and cropproduction under stressed condition. In this study, we investigated osmo and hydropriming effect in two salt stressed chickpeacultivars. This research monitors the changes in growth factors, proline content, lipid peroxidation and H2O2 scavenging enzymesactivities in osmo (mannitol 4%) and hydro (water) primed plantlets of two salt stressed chickpea cultivars (Arman and Biv...
Autori: Akbar Mostajeran, Fatemeh Khodabakhsh, Rayhaneh Amooaghaie
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=26315

AbstractDue to its toxicity, persistence in the environment and specific properties, 1,2-dichlorobenzene (DCB) has been listed as apriority pollutant by the European Agency. In this study, the oxidation with ozone alone as well as in combination withultraviolet irradiation was applied for the degradation of DCB. This article presents the abatements obtained, after the varioustreatments proposed, in the levels of chemical pollution as monitored by chromatographic techniques (GC/ECD, GC/MS andHPLC...
Autori: Bertrand Sancey, Corina Bradu, Grégorio Crini
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=26328

AbstractLead (II) can bind with papain at its active site, destroying the enzymatic activity. Papain a sulfhydryl protease has beenimmobilized in calcium alginate bead for the purpose of removal of lead (II) from industrial waste water. Effect of lead (II) onthe enzyme activity of immobilized papain, has been observed. A detailed kinetic study has been carried out and the data arefitted to different kinetic models. EDS (Energy Dispersive X-Ray Spectrometry) and desorption study suggest that meta...
Autori: Aparupa Bhattacharyya, Parameswar De, Soumasree Chatterjee, Srabanti Basu, Susmita Dutta
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=26338

AbstractAccording to the study results, the level of soil pollution with heavy metals in the northern part of Kryvyy Rih iron ore basin(Kryvyy Rih district, Ukraine) reach to “moderately threating”. In the southern part of Kryvyy Rih basin (Shyroke district,Ukraine) the level of soil pollution was between two assessments: “moderately threating” and “permissible”. The results of thestudy on buffer capacity of usual black soils show that the tested elements can be arranged in the following descend...
Autori: Mykola Kharytonov
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=26389

AbstractTetracycline (TC) are used as bacteriostatic agents, inhibits the synthesis of bacterial proteins, with activity against awide variety of organisms with application in human and veterinary medicine. The presence of TC in theenvironment constitutes a serious problem, being weakly metabolized or absorbed into the human or animal bodycon. TC residues even in low concentration may produce serious toxic and other effects on humans and livingorganism. In this study are presented the applicatio...
Autori: Carmen Ionela Brînzilă
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=26513

Abstract Due to its very low moisture content, the solid waste from the two-phase olive oil extraction process (dry olive mill residue, DOR) can be easily stored and used as a readily available basal liquid culture medium via its reconstitution with water. Five white-rot fungi (namely Phanerochaete chrysosporium NRRL 6361, Lentinus tigrinus CBS 577.79, Pleurotus pulmonarius CBS 664.97, Phlebia radiata DABAC 9 and Botryosphaeria rhodina DABAC P82), previously selected in a screening program, ...
Autori: Alessandro D’Annibale
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=26598

Abstract Molinate (S-ethyl-azepane-1-carbothioate) is a thiocarbamate herbicide used in rice cultivation for the control of grass weeds. Environmental contamination with molinate is of major concern due to the adverse effects described both for humans and animals. Molinate hydrolase, a novel amidohydrolase previously characterized, is responsible for the initial breakdown of molinate, cleaving the thioester bond of molinate, releasing ethanethiol and azepane-1-carboxylate (ACA). Biotechnolo...
Autori: Ana M. Paiva
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=26642

Abstract β-galactosidase from Aspergillus oryzae was strongly immobilized on magnetic particles functionalized with amino groups. By simple incubation without any activating agents, electrostatic interactions between amino groups and enzymes allowed obtaining a strong linkage. The immobilization efficiency was studied with the quantification of amino groups of the particles and of immobilized β-galactosidase. Kinetic parameters, especially the maximal velocity Vmax and the affinity...
Autori: Kalim Belhacene
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=26682

Abstract The present study explores the potential of Streptococcus epidermis, an indigenous isolate for biotransformation of phenol and phenol derivatives. Different phenolic derivatives such as 4-methylphenol, methylbenzene, 2,4-dichlorophenol, 2,6- dichlorophenol and 4-nitrophenol were attempted for biotransformation study. The results have shown that 4-nitrophenol was best transformed. The effect of degradation parameters like pH and temperature was studied, showing the value of 7.0 as o...
Autori: Bhavana V. Mohite
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=26705

Abstract Total anthocyanins were extracted from six sweet cherry cultivars grown in Sakarya, Turkey. In vitro inhibition effects of the extracted total anthocyanins on purified carbonic anhydrase I and II were investigated using CO2 as a substrate. All the extracted total anthocyanins inhibited the activity of the human carbonic anhydrase (hCA) I and II enzymes. Among all the cultivars, ‘Karakiraz’ was found to be the most active on both hCA I (IC50 = 30.59 μg/mL) and hCA II a...
Autori: Taki Demir
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=26715

Abstract Natural herbicides may be more sustainable than their chemical homologues, but they are still toxic substances that can affect humans and animals. Implementation of natural herbicides may require just as many safety precautions as the application of commercial herbicides, hence the need not to confuse the term "organic" with "non toxic". Allelopathy is accomplished through chemical inhibition of species towards each other, being an antagonistic phenomenon. Respon...
Autori: Ana Maria Vîrteiu
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=26752

Abstract Much attention is paid to removing formaldehyde from indoor air to decrease the health risk for urban inhabitants since formaldehyde is a main indoor pollutant causing various symptoms. In this study, formaldehyde removal by the shoots of potted Chlorphytum comosum (spider plant), Epipremnum aureum (golden pothos) and Aloe vera (aloe) was investigated with a dynamic chamber technique. The results indicated that the order of formaldehyde removal capacities for the plant shoots was C....
Autori: Haiping Hou
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=26908

The pickling liquor used in the stainless steel manufacturing process is a mixture of nitric and hydrofluoric acid which generates wastewater with nitrate concentrations ranging between 500 and 6000 mgNO3--N/L. In the present study, laboratory-scale anoxic Sequencing Batch Reactors (SBRs) were used to treat high nitrate wastewaters. Two different sludge inocula were tested: one from a Municipal Solid Waste Leachate Treatment Plant (LTP), and the other from a Sewage Treatment Plant (STP). Meth...
Autori: Ana Isabel Pelaez
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=26933

Environmental persistence of endocrine disrupting estrogen chemicals (EDC) subsequent to endocrine function even at nanogram concentration in environmental matrices has garnered significant global attention due to their possible disruptive effects in an intact organism, or its progeny. An attempt was made in present study to investigate the occurrence of two estrogens viz. natural (estriol, E3) and synthetic (17α-ethynylestradiol, EE2) in the surface waster bodies of Hyderabad, India. A...
Autori: A. Kiran Kumar
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=26953

In the presence of surfactant sodium dodecylbenzene sulfonate (SDBS), the development of main hydrolysis products (soluble carbohydrate and protein) and hydrolytic enzyme activities (amylase and protease) were monitored during microaerobic thermophilic digestion (MATD) of waste activated sludge (WAS). Results showed that the presence of SDBS remarkably increased the solubility of protein and carbohydrate into aqueous phase. The maximal concentration of carbohydrate and protein was 990.39 and...
Autori: Qi Yang
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=26958

The release of low molecular weight (LMW) organic acids under lead (Pb) and zinc (Zn) stresses in Typha latifolia (T. Latifolia) and Vetiver zizanioides (V. zizanioides) were investigated. In addition, changes in antioxidative enzymes: superoxide dismutase (SOD) and peroxidase (POD) in the leaves of T. Latifolia under Pb stress were also examined. Amongst the eight types of LMW organic acids detected in the present study, oxalic acid was found to be the most dominant during Pb and Zn treatmen...
Autori: Yu Bon Man
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27092

Biological control relying on soil microorganisms may offer feasible and sustainable perspectives in many agrosystems for management of soil pests such as root-knot nematodes (RKNs, Meloidogyne spp.). The rhizosphere environment shows a complex of soil microbial communities, including beneficial organisms such as specialized bacterial pathogens and/or of rhizosphere fungi. New advanced technologies like Next Generation Sequencing (NGS) may enlarge our knowledge about the biodiversity and rol...
Autori: Aurelio Ciancio
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27109

This work focuses on treatment of lead polluted waste glass from urban collection (Pb≈5% wt) for its valorization as support in the immobilization of lipase, because soda-lime glasses are used in enzymatic catalysis. First of all, an extraction process of surface Pb was performed. The process is based on nitrilotriacetic acid (NTA) chelating agent. The operating conditions are: T=80°C, t=1h, pH 10, solid/liquid weight ratio=1/10, reagent concentration=0.1 M. Leaching tests at contr...
Autori: Luisa Barbieri
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27122

Rapeseed meal (RM) is an abundant and cheap by-product of oil extraction process. RM is a rich source of nitrogen, carbon, and minerals. Thanks to these features, RM has a striking potential for eco-friendly production of high value products in biotechnological processes. In this study, the possibility of using a low-cost medium based on the water soluble fraction of RM for laccase production by the fungus Pleurotus ostreatus has been evaluated and compared with the use of synthetic complex ...
Autori: Lucia Guarino
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27129

The aim of the present study was to prepare environmentally friendly silver-based materials using Cornus mas L. fruits extract. For this purpose, silver nanoparticles (Cornus mas-AgNPs) were green synthesized using this plant extract. The bioreduction of silver ions was analyzed by UV–VIS and ATR-FTIR spectroscopic studies. Scanning Electron Microscopy (SEM) analysis revealed the formation of spherical phyto-nanosilver particles with size ranging between 12 and 40 nm. Different amounts ...
Autori: Ioana-Raluca Suica-Bunghez
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27139

Safe utilization of fly ash (FA) and reclamation of sodic soils are important environmental problems. In this study a field experiment was carried out with Vigna radiata L. crop using fly ash (FA) and phosphogypsum (PGY) as amendments in sodic soil during 2011 at Lucknow, Uttar Pradesh, India. V. radiata L. was sown in sodic soil amended with press mud (10 t ha-1) considered as control. Besides, 10 and 20 t ha-1 doses of FA and PGY were considered as other four treatments, respectively. The grow...
Autori: Kripal Singh
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27180

In this paper low power microwave radiation impact on Zea mays root meristeme tissues was investigated. Seeds with uniform genophond were exposed for 3 hours at 10 W incident power level in controlled condition to continuous wave at three different frequencies (0.6 GHz, 1 GHz and 2.5 GHz) and let to germinate after electromagnetic exposure. Dosimetric assessment made experimentally and computationally allowed the proper energetic characterization of the interaction between vegetal tissue and rad...
Autori: Mihaela Răcuciu
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27196

With population growth and increasing industrialization, enormous amounts of agro-industrial wastes are being produced. Without proper use, these keep piling up leading to tremendous pressure on environmental safety. There is also considerable economic interest not only in the proper utilization of these wastes, but also in developing appropriate processes for this purpose. Sludge generated in pulp and paper mills is one of such solid wastes. Although several studies have investigated its reuse,...
Autori: Patrícia Raquel Silva Zanoni
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27320

The monomer with ketimine side group, 2-(1-(benzylimino)ethyl)phenol (2-BEP), was synthesized from the condensation of 2- hydroxyacetophenone and benzyl amine. The enzymatic oxidative polymerization of 2-BEP was performed in the presence of hydrogen peroxide using horseradish peroxidase (HRP) as catalyst. The oxidation reaction was carried out in various solvents and phosphate buffers at room temperature. These studies have shown that a dark brown polymer was successfully synthesized by utilizin...
Autori: Senem Tapan
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27377

The utilization of agro-industrial waste as a potential substrate for producing enzymes may serve a dual purpose of reducing the environmental pollution along with producing a high value commercial product. Twelve different agro-industrial wastes were evaluated for extracellular BGL (β-glucosidase) production by a mutant of Bacillus subtilis on solid state fermentations (SSF). The Citrus sinensis bagasse was found to be the most suitable substrate with highest BGL titer (35 U/gds). Optimum ...
Autori: Ruchi Agrawal
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27378

The kinetics of azo dye reduction based on combined effect of biochemical reduction by anaerobic mixed culture and chemical reduction with hydrogen sulfide was investigated. Hydrogen sulfide was produced by sulphate reducing bacteria existing in the anaerobic mixed culture. The effect of initial anaerobic biomass, initial substrate, and initial dye (Reactive Orange 107) concentrations on reduction kinetics were studied with batch assays. The municipal waste sludge was used as electron donating s...
Autori: Umay Gokce Ozkan-Yucel
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27386

• EXPERIMENTAL INVESTIGATION OF TEMPERATURE EFFECTS ON THE PARAMETERS OF PHOTOVOLTAIC PANELS    • CONCENTRATIONS OF CHROMIUM, CADMIUM AND NICKEL IN TWO CONSUMED FISH SPECIES OF PERSIAN GULF, IRAN    • IMPROVING THE ENERGY EFFICIENCY OF RESIDENTIAL BUILDINGS BY USING A DRAIN WATER HEAT RECOVERY SYSTEM    • ASSESSMENT OF METAL CONTAMINATION AND ECOLOGICAL RISK IN URBAN SOILS SITUATED NEAR A METALLURGICAL COMPLEX    • FINANCIAL DE...
Autori:
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27398

EXPERIMENTAL INVESTIGATION OF TEMPERATURE EFFECTS ON THE PARAMETERS OF PHOTOVOLTAIC PANELS    • CONCENTRATIONS OF CHROMIUM, CADMIUM AND NICKEL IN TWO CONSUMED FISH SPECIES OF PERSIAN GULF, IRAN    • IMPROVING THE ENERGY EFFICIENCY OF RESIDENTIAL BUILDINGS BY USING A DRAIN WATER HEAT RECOVERY SYSTEM    • ASSESSMENT OF METAL CONTAMINATION AND ECOLOGICAL RISK IN URBAN SOILS SITUATED NEAR A METALLURGICAL COMPLEX    • FINANCIAL DEVELOPME...
Autori:
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27399

Lignocellulosic residues are relatively recalcitrant to bioconversion during anaerobic digestion (AD) for biogas production. Pre-treatments with cellulolytic enzymes or diluted alkali can facilitate biomass hydrolysis and enhance the process. Both pre-treatments require low energy and chemical inputs, without accumulation of inhibitor. Milled wheat straw was pre-treated with hydrolytic enzymes or with diluted NaOH before AD. The pre-treatments were performed on sterilized, stabilized with formic...
Autori: Ciro Vasmara
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27420

This paper reports an improved chlorolignin (CL) oxidative decolorization in alkaline media by the use of a heterogeneous system consisting of hydrogen peroxide and a silica [bis(dibenzoylmethido) copper II] (S1) catalyst. The catalytic functions of S1 in the presence of H2O2 were evaluated by comparing the chlorolignin decolorization in media with and without addition of catalyst. Since decolorization is associated with CL degradation, the pH variation and chloride release assays were carrie...
Autori:
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27448

A total of 152 different actinomycetes isolates were recovered from samples that were collected from different ecosystems of Algeria. The desert soil of Bousaada gives the greatest number of actinomycetes compared to others soils. The first screening indicates that 63.15 % of the isolates were able to produce carboxymethyl cellulase enzyme. Isolates were evaluated for their cellulase activity by growing in carboxymethyl cellulose broth. B-PNG23 isolate is one of the isolates displayed a high enz...
Autori: Azzeddine Bettache
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27451

The delignification of wood pulp using hydrogen peroxide and xylanase enzyme in an ultrasonicator was studied at different process conditions. The factors influencing the chemical delignification were studied simultaneously using the Box-Behnken design of experiments. The mathematical model for Kappa reduction as functions of consistency of the pulp, concentration of peroxide and the temperature of delignification was developed. The response optimization studies for minimizing the Kappa numb...
Autori: Palaniappan Ramesh
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27480

A large number of microorganisms are capable of producing cellulases; however, fungi are considered among the most active enzymes producers. Different strains of white rot fungi (WRF) were reported to have a great potential for biotechnological applications and cellulase enzymes is much involved in this effect, especially for bioethanol production. Misiones is included in a forest region characterized by a vast biodiversity, and within this environment, a large number of microorganisms can be...
Autori: Ernesto M. Giorgio
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27489

The present study deals with the production of biogas from post-harvest wastes of pineapple such as peels, leaves, mixture of peels and leaves with the two different types of inoculum viz., Cow Manure (CM) and Novel microbial Consortia (NC). The results between the two types of inoculum were compared to find out their efficiency. Among the different substrate-inoculum combinations, the biogas production potential was evaluated which resulted that NC was more efficient compared to CM. All assays ...
Autori: Mukesh Kumar
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27496

Plant growth promoting rhizobacteria (PGPR) serve as an alternative tool in sustainable agriculture. PGPR influence the heavy metal accumulation of crops in contaminated soils, either by decreasing or increasing the accumulation. This study focuses on the effect of plant growth-promoting rhizobacteria on the heavy metal uptake, plant growth and stress response of bean plants. The simultaneous treatment of Cd2+ and Mitsuaria chitosanitabida T310-2/4 (PGPR strain) as well as Zn2+ and bacteria resu...
Autori: Éva Boglárka Vincze
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27554

Plant growth promoting rhizobacteria (PGPR) colonize rhizosphere and enhance growth in crop plants, by producing various regulatory substances. Since PGPR employ diverse array of mechanisms and exact mechanism and role are still to be established. Therefor experiment was designed 1) to identify and 2) to select potential rhizobacterial strains based on their impact on the growth, physiological and enzymatic activities of rice. A CRD pot experiment with six PGPR isolates; two containing only ACC-...
Autori: Waseem Hassan
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27587

β-glucosidases are very important in the improvement of cellulose degradation rates by diminishing the inhibition of end products. Although, cellulase cost has reduced significantly in last decade but still the high costs of biomass saccharification is one of the main challenges for the commercialization of ethanol from LC biomass. In this work, β-glucosidase immobilization in calcium alginate was optimized using response surface methodology. 89% immobilization efficiency was achieved ...
Autori: Ruchi Agrawal
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27614

FLUCTUATION OF RIVER NETWORK WATER ENVIRONMENTAL CARRYING CAPACITY IN A COMPLICATED RIVER-LAKE SYSTEM    • MINERALOGY AND GEOCHEMICAL DISTRIBUTION OF HEAVY METALS FOR THE FAGUL CETĂȚII TAILINGS, ROMANIA    • ACCUMULATION AND AVAILABILITY OF TRACE ELEMENTS FROM SOIL INTO ORIENTAL TOBACCO GROWN IN MACEDONIA    • OLFACTORY EFFECT ON LANDSCAPE PREFERENCE    • EFFECT OF MOLASSES ADDITION AS BIODEGRADABLE MATERIAL ON PHENOL REMO...
Autori:
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27628

The enzyme-based biosensor stability and its analytical performance depend on both the immobilization process and the matrix used for immobilization of the enzyme into a stable layer. In this paper, the development and evaluation of an optimal enzymatic layer for stable encapsulation of the acetylcholinesterase enzyme (AChE) through the sol-gel method onto mediated carbon-ink screen-printed electrodes was performed, obtaining electrochemical enzyme-based biosensors. The enzyme was immobilized on...
Autori: Melinda David
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27636

α-cypermethrin is a pyrethroid and environmental genotoxic pesticide extensively used worldwide for agricultural applications. However, like other pesticides, it affects non-target organisms as well as target organisms and causes various toxic effects. To evaluate α-cypermethrin toxicity in non-target organisms, the effect of α-cypermethrin on the activity of malondialdehyde (MDA, an indicator of lipid peroxidation) content, antioxidant enzymes (superoxide dismutase, SOD; perox...
Autori: Ozkan Aksakal
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27647

Though Scirpus triqueter plays an important role in protecting the ecological functions of Yangtze River estuary wetland which is frequently contaminated by petroleum, knowledge is limited about the mechanisms involved. In this study, comparative analysis of soil properties, microbial community structure and soil enzymes in the rhizosphere of S. triqueter and bulk soil was conducted throughout one year to provide an insight into the phytoremediation mechanism in the field. The total petroleum hy...
Autori: Jing Wei
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27697

Application of a biological treatment system using white rot fungi is an interesting alternative for treatment of water and wastewater contaminated with persistent organic pollutants, such as polycyclic aromatic hydrocarbons (PAHs). A packed bed bioreactor using the white rot fungi, Phanerochaete chrysosporium (P. chrysosporium) was evaluated for degradation of pyrene and phenanthrene in polluted wastewater. White rot fungi have significant potential to metabolize organic pollutants such as PAHs...
Autori: Mahsa Baniasadi
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27767

The aim of this paper was to investigate and understand the relationship between several physical and chemical parameters such as temperature, chemical oxygen demand (COD), and biochemical oxygen demand (BOD) on the one hand and dynamics of bacterial populations involved in the breakdown (hydrolysis) of macromolecular organic matter, on the other. The study was carried out during July-November 2009 at Cernavoda Wastewater Treatment Plant and included quantitative estimation of proteolytic, amylo...
Autori: Constantin Ioan
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27872

Indigenous Pseudomonas veronii 2E, Klebsiella oxytoca P2 and Klebsiella ornithinolityca 1P were tested as catalysts for the transformation of Cr(VI) to Cr(III) for Cr(VI)-containing wastewater biotreatments. The Cr(VI) biotransformation was assayed during bacterial growth and by using pre-grown quiescent cells. Proof of the unsuitability of a biotreatment based on Cr(VI)-reduction during bacterial growth was found: the reduction rate and the maximum Cr(VI) concentration able to be reduced were t...
Autori: Mauricio Javier Alessandrello
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27853

Tunisian fish-processing industries generates large amounts of by-products. Their disposal is an increasing environmental and health problem. For an economic utilization, these by-products may be used in producing higher value-added products such as protein hydrolyzates. In this study, protein hydrolyzates with different degrees of hydrolysis (12%, 20% and 26%) were prepared from heads and viscera of Sardinella aurita using crude protease of Bacillus licheniformis NH1. The obtained hydrolyzates ...
Autori: Nabil Souissi
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=27852

Since the middle of the past century, when the synthetic fibers began their rise, wool has steadily lost importance in the textile sector, and now is a marginal fiber on the textiles market. Great amounts of non-marketed raw wool have turned from an income source for sheep farmers into a problematic waste, which is, at best, burn or landfill. With increasing interest for sustainable use of natural resources, wool was reconsider as an underrated, underused renewable resource, worthy of a bette...
Autori: Hossein Rajabinejad
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=28031

Sediments in coastal areas can accumulate a variety of contaminants, acting as both carriers and long-term secondary sources of contamination for aquatic ecosystems. Nowadays, there is a growing interest on developing new assessment criteria of sediment ecological quality for setting priorities and management strategies of contaminated materials. According to the literature, the weight of evidence (WOE) approach has been developed to provide a multidisciplinary characterization which combines di...
Autori: Francesco Todaro
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=28089

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Autori: Muhammad Akram
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=28136

Growth rate and biomass yield in the case of Arthrospira platensis depend on nutrient availability, temperature and light, which can determine changes in the metabolism and therefore in the biomass composition. The aim of this study is to evaluate the behavior of A. platensis during the growing time in several liquid culture media and their influence on the yield of biomass production. The following culture media have been investigated in this sense: ARS, Zarrouk (Z), modified Zarrouk (MZ), eco...
Autori: Mariana Diaconu
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=28163

Silk sericin is a water-soluble macromolecular protein obtained from the raw cocoons of silkworm, Bombyx mori. It comprises of about 25% of the total cocoon shell weight and is categorized as a waste by-product in textile and silk industries after the extraction of silk fibers. This study emphasizes on the extraction of sericin from B. mori by a conventional salt alkaline method and characterized their physico-chemical properties, thermal stability, anti-bacterial and anti-oxidant activities. T...
Autori: Inderchand Manjubala
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=28213

Nutrients have been considered the main factor affecting microorganisms responsible for methane gas production during anaerobic digestion processes. In this study, the nutritional composition of some fruit wastes; mango (M), watermelon (W), pawpaw (P), their effects on methane gas production, and resulting energy values were assessed. Substrate samples for anaerobic digestion where prepared into various slurry treatments; M, W, P, WM, PW, MP, MWP and CONTROL. Proximate compositions of the subst...
Autori: Ogemdi Chinwendu Anika
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=28233

In the wake of advance industrialization, the intensive growth of modern textile production and inappropriate wastewater treatment strategies have led to the release of noxious and carcinogenic contaminants like azo dyes directly or indirectly into the environment. Therefore, to ensure the protection of the humankind and natural bionetwork, cost-effective and efficiently regulated control measures are necessary. On this account, recent developments in biotechnology and microbiology have driven ...
Autori: Geetanjali Rajhans
ISSN: 1843 - 3707
http://www.ecozone.ro/reviste.php?RID=28318